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DDIT3 / CHOP Polyclonal Antibody (A0221)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0221)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A0221)
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)
Review
ABclonal: review for DDIT3 / CHOP Polyclonal Antibody(A0221)

Overview

Product nameDDIT3 / CHOP Polyclonal Antibody
Catalog No.A0221
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 / CHOP (NP_004074.2).
SequenceGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA
Gene ID1649
Swiss protP35638
SynonymsDDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DDIT3 / CHOP
Calculated MW19kDa/21kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, C2C12, C6
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human, Mouse, Rat)

IHC(Rat)

WB(Rat)

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0221) }

Western blot - DDIT3 / CHOP Polyclonal Antibody (A0221)

Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (A0221) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A0221) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A0221)

Immunohistochemistry of paraffin-embedded rat testis using DDIT3 / CHOP antibody (A0221) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221) }

Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)

Immunofluorescence analysis of L929 cells using DDIT3 / CHOP antibody (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221) }

Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)

Immunofluorescence analysis of C6 cells using DDIT3 / CHOP antibody (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221) }

Immunofluorescence - DDIT3 / CHOP Polyclonal Antibody (A0221)

Immunofluorescence analysis of U2OS cells using DDIT3 / CHOP antibody (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

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