Product Type > Antibodies > Tag and Loading Control Antibodies

DDDDK-Tag Rabbit mAb (AE063)

Publications (55) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - DDDDK-Tag Rabbit mAb (AE063)

Western blot analysis of lysates from 293T cells, using DDDDK-Tag Rabbit mAb (AE063) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag Rabbit mAb (AE063). Green: GFP expression. Blue: DAPI for nuclear staining. 

ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using DDDDK-Tag Rabbit mAb (AE063) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063), Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg DDDDK-Tag antibody (AE063). Western blot was performed from the immunoprecipitate using DDDDK-Tag (AE063) at a dilution of 1:1000.

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Overview

Product name DDDDK-Tag Rabbit mAb
Catalog No. AE063
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5111-02

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Immunogen information

Immunogen A synthetic peptide corresponding to DDDDK tag.
Sequence DYKDDDDK
Gene ID
Swiss prot
Synonyms DDDDK; DDDDK tag; DDDDK-tag; DDDDK-Tag
Calculated MW
Observed MW Refer to Figures

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples 293T
Cellular location
Customer validation

WB (Oreochroms mossambcus, Mus musculus, Homo sapiens, Arabidopsis thaliana, Bombyx mori Linnaeus, Other)

IHC (Mus musculus, Homo sapiens)

IF (Mus musculus, Homo sapiens, Other)

ChIP (Arabidopsis thaliana)

IP (Arabidopsis thaliana, Homo sapiens, Other, Mus musculus)

Co-IP (Glycine max L, Homo sapiens)

RIP (Homo sapiens)

FC (Homo sapiens)

CO-IP (Homo sapiens)

ELISA (Other)

Documents

Certificate of Compliance

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Lot number

DDDDK-Tag Rabbit mAb images

ABclonal:Western blot - DDDDK-Tag Rabbit mAb (AE063)}

Western blot - DDDDK-Tag Rabbit mAb (AE063)

Western blot analysis of lysates from 293T cells, using DDDDK-Tag Rabbit mAb (AE063) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)}

Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag Rabbit mAb (AE063). Green: GFP expression. Blue: DAPI for nuclear staining. 
ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)}

Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using DDDDK-Tag Rabbit mAb (AE063) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)}

Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063), Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)}

Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg DDDDK-Tag antibody (AE063). Western blot was performed from the immunoprecipitate using DDDDK-Tag (AE063) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK

* Data provided by citexs.com, for reference only.

Publishing research using AE063? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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