Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Cytokeratin 5 (CK5) Rabbit mAb (A11396)

PathoQIHC Pathology

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Human skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Mouse skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

You may also interested in:

Overview

Product name Cytokeratin 5 (CK5) Rabbit mAb
Catalog No. A11396
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0585

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647).
Sequence ISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
Gene ID 3852
Swiss prot P13647
Synonyms K5; KRT5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A; Cytokeratin 5 (CK5)
Calculated MW 62kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IF/ICC HumanMouseRat
IHC-P Human
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples A431 cells, Mouse liver, Rat liver, Rat testis
Cellular location cytoplasm, cytosol, extracellular exosome, nucleus
Customer validation

WB (Homo sapiens)

IHC (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Cytokeratin 5 (CK5) Rabbit mAb images

ABclonal:Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Western blot - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunohistochemistry - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Human skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Mouse skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)}

Immunofluorescence - Cytokeratin 5 (CK5) Rabbit mAb (A11396)

Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Inquire About This Product

Submit your question about A11396 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on KRT5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KRT5. (Distance between topics and target gene indicate popularity.) KRT5

* Data provided by citexs.com, for reference only.

Publishing research using A11396? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order