Products > Catalog Antibodies > Monoclonal Antibodies

Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunofluorescence analysis of rat skin using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunofluorescence analysis of human skin using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCytokeratin 5 (KRT5) Rabbit mAb
Catalog No.A11396
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647).
SequenceISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
Gene ID3852
Swiss protP13647
SynonymsCK5; DDD; DDD1; EBS2; K5; KRT5A
Calculated MW62kDa
Observed MW62KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-431, Mouse liver, Rat liver, Rat testis
Cellular location
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)}

Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)}

Western blot - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)}

Immunohistochemistry - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)}

Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunofluorescence analysis of rat skin using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)}

Immunofluorescence - Cytokeratin 5 (KRT5) Rabbit mAb (A11396)

Immunofluorescence analysis of human skin using Cytokeratin 5 (KRT5) (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A11396 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A11396? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order