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[KO Validated] Cytochrome c Rabbit pAb (A13430)

Publications (14) Datasheet

ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)
ABclonal:Immunofluorescence - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Overview

Product name[KO Validated] Cytochrome c Rabbit pAb
Catalog No.A13430
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
SequenceMGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE
Gene ID54205
Swiss protP99999
SynonymsCYCS; CYC; HCS; THC4; cytochrome c
Calculated MW11kDa
Observed MW11KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SH-SY5Y, BT-474
Cellular locationMitochondrion intermembrane space
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Andrias davidianus, Giardia duodenalis)

ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Western blot analysis of extracts of various cell lines, using Cytochrome c Rabbit pAb (A13430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Western blot analysis of extracts from normal (control) and Cytochrome c knockout (KO) 293T cells, using Cytochrome c antibody (A13430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded rat lung using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded human tonsil using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded human liver cancer using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunofluorescence - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (A13430) at dilution of 1:100. Blue: DAPI for nuclear staining.

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