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Cyclin E1 Rabbit pAb (A14225)

Publications (12) Datasheet

ABclonal:Western blot - Cyclin E1 Rabbit pAb (A14225)

Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (A14225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Cyclin E1 Rabbit pAb (A14225)

Immunohistochemistry of paraffin-embedded mouse lung using Cyclin E1 Rabbit pAb (A14225) at dilution of 1:150 (40x lens).

Overview

Product nameCyclin E1 Rabbit pAb
Catalog No.A14225
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human Cyclin E1 (NP_001229.1).
SequenceAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
Gene ID898
Swiss protP24864
SynonymsCCNE1; CCNE; pCCNE1; cyclin E1
Calculated MW47kDa
Observed MW47KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMCF7, K-562, Mouse brain, Rat brain
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Capra hircus, Mus musculus)

ABclonal:Western blot - Cyclin E1 Rabbit pAb (A14225) }

Western blot - Cyclin E1 Rabbit pAb (A14225)

Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (A14225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Cyclin E1 Rabbit pAb (A14225) }

Immunohistochemistry - Cyclin E1 Rabbit pAb (A14225)

Immunohistochemistry of paraffin-embedded mouse lung using Cyclin E1 Rabbit pAb (A14225) at dilution of 1:150 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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