Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Cyclin D1 Rabbit pAb (A0310)

Review (1) Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Cyclin D1 Rabbit pAb (A0310)

Western blot analysis of extracts of rat kidney, using Cyclin D1 antibody (A0310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Cyclin D1 antibody (A0310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Cyclin D1 antibody (A0310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for Cyclin D1 Rabbit pAb(A0310)

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Overview

Product name Cyclin D1 Rabbit pAb
Catalog No. A0310
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 151-271 of human Cyclin D1 (NP_444284.1).
Sequence NLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAA
Gene ID 595
Swiss prot P24385
Synonyms BCL1; PRAD1; U21B31; D11S287E; Cyclin D1
Calculated MW 34kDa
Observed MW 34kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Rat kidney
Cellular location Cytoplasm, Membrane, Nucleus
Customer validation

WB (rat cellsMus musculus, Mus musculus, Homo sapiens)

IHC (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Cyclin D1 Rabbit pAb images

ABclonal:Western blot - Cyclin D1 Rabbit pAb (A0310)}

Western blot - Cyclin D1 Rabbit pAb (A0310)

Western blot analysis of extracts of rat kidney, using Cyclin D1 antibody (A0310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)}

Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Cyclin D1 antibody (A0310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)}

Immunohistochemistry - Cyclin D1 Rabbit pAb (A0310)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Cyclin D1 antibody (A0310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCND1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCND1. (Distance between topics and target gene indicate popularity.) CCND1

* Data provided by citexs.com, for reference only.

Publishing research using A0310? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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