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Cyclin B1 Polyclonal Antibody (A2056)

Reviews (8)Publications (5) Datasheet

ABclonal:Western blot - Cyclin B1 Polyclonal Antibody (A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)
Review
ABclonal: review for Cyclin B1 Polyclonal Antibody(A2056)

Overview

Product nameCyclin B1 Polyclonal Antibody
Catalog No.A2056
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1).
SequenceTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
Gene ID891
Swiss protP14635
SynonymsCCNB1; CCNB; cyclin B1
Calculated MW44kDa/48kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, PC-3
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

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WB(Rat, Human)

ABclonal:Western blot - Cyclin B1 Polyclonal Antibody (A2056) }

Western blot - Cyclin B1 Polyclonal Antibody (A2056)

Western blot analysis of extracts of various cell lines, using Cyclin B1 Antibody (A2056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

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