Products > Catalog Antibodies > Secondary Antibodies

Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)

Publications (36) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Cy3 Goat Anti-Rabbit IgG (H+L)
ABclonal:Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)

Immunofluorescence analysis of HeLa cells, using DiMethyl-Histone H3-K4 antibody (A2356) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007).
Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)

Immunofluorescence analysis of HeLa cells, using BCL2 antibody (A2845) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007).
Blue: DAPI for nuclear staining.

Overview

Product nameCy3 Goat Anti-Rabbit IgG (H+L)
Catalog No.AS007
Host speciesGoat
Purification methodAffinity purification
IsotypeCy3 conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • IF 1:100 - 1:800
  • FC 1:100 - 1:800
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: 50% glycerol, pH7.3.
Application keyImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Rattus norvegicus, Mus musculus, Homo sapiens, Tegillarca granosa, Danio rerio, Sus scrofa)

IHC(Rattus norvegicus)

WB(Mus musculus)

    ABclonal:Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)}

    Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)

    Immunofluorescence analysis of HeLa cells, using DiMethyl-Histone H3-K4 antibody (A2356) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007).
    Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)}

    Immunofluorescence - Cy3 Goat Anti-Rabbit IgG (H+L) (AS007)

    Immunofluorescence analysis of HeLa cells, using BCL2 antibody (A2845) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007).
    Blue: DAPI for nuclear staining.

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