Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CtBP1 Rabbit pAb (A1707)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - CtBP1 Rabbit pAb (A1707)

Western blot analysis of lysates from mouse brain, using CtBP1 Rabbit pAb (A1707) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

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Overview

Product name CtBP1 Rabbit pAb
Catalog No. A1707
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human CtBP1 (NP_001319.1).
Sequence MNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDF
Gene ID 1487
Swiss prot Q13363
Synonyms BARS; HADDTS; CtBP1
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse brain
Cellular location Cytoplasm, Nucleus
Customer validation

Co-IP (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

CtBP1 Rabbit pAb images

ABclonal:Western blot - CtBP1 Rabbit pAb (A1707)}

Western blot - CtBP1 Rabbit pAb (A1707)

Western blot analysis of lysates from mouse brain, using CtBP1 Rabbit pAb (A1707) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

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Related Targets

Based on existing publications, the following genes are closely related to research on CTBP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CTBP1. (Distance between topics and target gene indicate popularity.) CTBP1

* Data provided by citexs.com, for reference only.

Publishing research using A1707? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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