Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Connexin 43 Rabbit pAb (A11752)

Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Connexin 43 Rabbit pAb (A11752)

Western blot analysis of extracts of various cell lines, using Connexin 43 antibody (A11752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Connexin 43 Rabbit pAb (A11752)

Immunofluorescence analysis of A-549 cells using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name Connexin 43 Rabbit pAb
Catalog No. A11752
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 233-382 of human Connexin 43 (NP_000156.1).
Sequence FKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
Gene ID 2697
Swiss prot P17302
Synonyms HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA; Connexin 43
Calculated MW 43kDa
Observed MW 43kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, Mouse heart, Rat heart
Cellular location Cell junction, Cell membrane, Endoplasmic reticulum, Multi-pass membrane protein, gap junction
Customer validation

IF (Mus musculus, Macaca fascicularis)

WB (Mus musculus, Homo sapiens, Sus scrofa)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Connexin 43 Rabbit pAb images

ABclonal:Western blot - Connexin 43 Rabbit pAb (A11752)}

Western blot - Connexin 43 Rabbit pAb (A11752)

Western blot analysis of extracts of various cell lines, using Connexin 43 antibody (A11752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)}

Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)}

Immunohistochemistry - Connexin 43 Rabbit pAb (A11752)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Connexin 43 Rabbit pAb (A11752)}

Immunofluorescence - Connexin 43 Rabbit pAb (A11752)

Immunofluorescence analysis of A-549 cells using Connexin 43 Rabbit pAb (A11752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GJA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GJA1. (Distance between topics and target gene indicate popularity.) GJA1

* Data provided by citexs.com, for reference only.

Publishing research using A11752? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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