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Complement C3 Rabbit pAb (A13283)

Publications (3) Datasheet

ABclonal:Western blot - Complement C3 Rabbit pAb (A13283)

Western blot analysis of extracts of HepG2 cells, using Complement C3 antibody (A13283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Complement C3 Rabbit pAb (A13283)

Immunohistochemistry of paraffin-embedded human prostate using Complement C3 antibody (A13283) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of rat liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of human liver cancer using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of mouse liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameComplement C3 Rabbit pAb
Catalog No.A13283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human Complement C3 (NP_000055.2).
SequenceKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN
Gene ID718
Swiss protP01024
SynonymsC3; AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1; HEL-S-62p; complement C3; C3 / C3a
Calculated MW187kDa
Observed MW115KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationSecreted
Customer validation

IHC(Rattus norvegicus)

WB(Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus)

IF(Mus musculus)

ABclonal:Western blot - Complement C3 Rabbit pAb (A13283) }

Western blot - Complement C3 Rabbit pAb (A13283)

Western blot analysis of extracts of HepG2 cells, using Complement C3 antibody (A13283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Complement C3 Rabbit pAb (A13283) }

Immunohistochemistry - Complement C3 Rabbit pAb (A13283)

Immunohistochemistry of paraffin-embedded human prostate using Complement C3 antibody (A13283) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283) }

Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of rat liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283) }

Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of human liver cancer using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Complement C3 Rabbit pAb (A13283) }

Immunofluorescence - Complement C3 Rabbit pAb (A13283)

Immunofluorescence analysis of mouse liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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