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Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Publications (5) Datasheet

ABclonal:Western blot - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Western blot analysis of extracts of Mouse brain, using Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunohistochemistry of paraffin-embedded human placenta using Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (A3795) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (A3795) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCollagen III alpha 1/COL3A1 Rabbit pAb
Catalog No.A3795
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1217-1466 of human COL3A1 (NP_000081.1).
SequenceAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL
Gene ID1281
Swiss protP02461
SynonymsCOL3A1; EDS4A
Calculated MW111kDa/138kDa
Observed MW200KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Mus musculus)

ABclonal:Western blot - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) }

Western blot - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Western blot analysis of extracts of Mouse brain, using Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) }

Immunohistochemistry - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunohistochemistry of paraffin-embedded human placenta using Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) }

Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (A3795) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795) }

Immunofluorescence - Collagen III alpha 1/COL3A1 Rabbit pAb (A3795)

Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (A3795) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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