Products > Catalog Antibodies > Polyclonal Antibodies

Claudin 1 Rabbit pAb (A2196)

Reviews (3)Publications (12) Datasheet

ABclonal:Western blot - Claudin 1 Rabbit pAb (A2196)

Western blot analysis of extracts of various cell lines, using Claudin 1 antibody (A2196) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Claudin 1 Rabbit pAb (A2196)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Claudin 1 Rabbit pAb (A2196) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Claudin 1 Rabbit pAb (A2196)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Claudin 1 Rabbit pAb (A2196) at dilution of 1:100 (40x lens).

Review
ABclonal: review for Claudin 1 Rabbit pAb(A2196)
Review
ABclonal: review for Claudin 1 Rabbit pAb(A2196)
Review
ABclonal: review for Claudin 1 Rabbit pAb(A2196)

Overview

Product nameClaudin 1 Rabbit pAb
Catalog No.A2196
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Claudin 1 (NP_066924.1).
SequenceMANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Gene ID9076
Swiss protO95832
SynonymsCLDN1; CLD1; ILVASC; SEMP1; claudin-1; Claudin 1
Calculated MW22kDa
Observed MW23KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, A-431
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, tight junction
Customer validation

(Mouse liver, Pig cells, Homo sapiens, Mus musculus)

WB(Rattus norvegicus, Mus musculus, Gallus gallus, Sus scrofa, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - Claudin 1 Rabbit pAb (A2196) }

Western blot - Claudin 1 Rabbit pAb (A2196)

Western blot analysis of extracts of various cell lines, using Claudin 1 antibody (A2196) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Claudin 1 Rabbit pAb (A2196) }

Immunohistochemistry - Claudin 1 Rabbit pAb (A2196)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Claudin 1 Rabbit pAb (A2196) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Claudin 1 Rabbit pAb (A2196) }

Immunohistochemistry - Claudin 1 Rabbit pAb (A2196)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Claudin 1 Rabbit pAb (A2196) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2196

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2196 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A2196? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order