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β-Catenin Rabbit pAb (A11932)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - β-Catenin Rabbit pAb (A11932)

Western blot analysis of extracts of various cell lines, using β-Catenin antibody (A11932) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - β-Catenin Rabbit pAb (A11932)

Western blot analysis of extracts of HCT116 cells, using β-Catenin antibody (A11932) at 1:1000 dilution.HCT116 cells treated with different concentrations of MUC-1.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - β-Catenin Rabbit pAb (A11932)

Immunofluorescence analysis of U2OS cells using β-Catenin antibody (A11932) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - β-Catenin Rabbit pAb (A11932)

Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug β-Catenin antibody (A11932). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A11932) at a dilition of 1:1000.

Review
ABclonal: review for β-Catenin Rabbit pAb(A11932)
Review
ABclonal: review for β-Catenin Rabbit pAb(A11932)

Overview

Product nameβ-Catenin Rabbit pAb
Catalog No.A11932
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human beta Catenin
SequenceEmail for sequence
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW9kDa/85kDa
Observed MW92kDa/100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T, Mouse brain, Mouse lung, Rat brain, HCT116
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

(Mouse colon)

WB(Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

ABclonal:Western blot - β-Catenin Rabbit pAb (A11932) }

Western blot - β-Catenin Rabbit pAb (A11932)

Western blot analysis of extracts of various cell lines, using β-Catenin antibody (A11932) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - β-Catenin Rabbit pAb (A11932) }

Western blot - β-Catenin Rabbit pAb (A11932)

Western blot analysis of extracts of HCT116 cells, using β-Catenin antibody (A11932) at 1:1000 dilution.HCT116 cells treated with different concentrations of MUC-1.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - β-Catenin Rabbit pAb (A11932) }

Immunofluorescence - β-Catenin Rabbit pAb (A11932)

Immunofluorescence analysis of U2OS cells using β-Catenin antibody (A11932) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - β-Catenin Rabbit pAb (A11932) }

Immunoprecipitation - β-Catenin Rabbit pAb (A11932)

Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug β-Catenin antibody (A11932). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A11932) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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