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Caspr1 Rabbit pAb (A10058)

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ABclonal:Western blot - Caspr1 Rabbit pAb (A10058)

Western blot analysis of extracts of various cell lines, using Caspr1 antibody (A10058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for Caspr1 Rabbit pAb(A10058)

Overview

Product nameCaspr1 Rabbit pAb
Catalog No.A10058
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1, 384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein, ' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human Caspr1 (NP_003623.1).
SequenceWGYYGCDEELVGPLYARSLGASSYYSLLTAPRFARLHGISGWSPRIGDPNPWLQIDLMKKHRIRAVATQGSFNSWDWVTRYMLLYGDRVDSWTPFYQRGHNSTFFGNVNESAVVRHDLHFHFTARYIRIVPLAWNPRGKIGLRLGLYGCPYKADILYFDGDDAISYRFPRGVSRSLWDVFAFSFKTEEKDGLLLHAEGAQGDYVTLELEGAHLLLHMSLGSSPIQPRPGHTTVSAGGVLNDQHWHYVRVDRFGRDVNFTLDGYVQRFILNGDFERLNLDTE
Gene ID8506
Swiss protP78357
SynonymsCNTNAP1; CASPR; CNTNAP; NRXN4; P190
Calculated MW156kDa
Observed MW170kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-937, PC-12, Mouse brain, Rat kidney
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

(rat cells)

WB(Rattus norvegicus)

IF(Rattus norvegicus)

ABclonal:Western blot - Caspr1 Rabbit pAb (A10058) }

Western blot - Caspr1 Rabbit pAb (A10058)

Western blot analysis of extracts of various cell lines, using Caspr1 antibody (A10058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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