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Caspase-9 Rabbit pAb (A2636)

Review (1)Publications (19) Datasheet

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded rat heart using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded human lung cancer using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody (A2636) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-9 Rabbit pAb(A2636)

Overview

Product nameCaspase-9 Rabbit pAb
Catalog No.A2636
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human Caspase-9
SequenceEmail for sequence
Gene ID842
Swiss protP55211
SynonymsCASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9; casp9; Caspase 9
Calculated MW17kDa/30kDa/36kDa/46kDa
Observed MW36kDa/46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, K-562, Mouse liver
Cellular location
Customer validation

(Human uterine stromal cells)

WB(Homo sapiens, Mus musculus, Giardia duodenalis)

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636) }

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636) }

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636) }

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded rat heart using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636) }

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded human lung cancer using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636) }

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636) }

Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody (A2636) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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