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Caspase-8 Rabbit pAb (A0215)

Reviews (2)Publications (22) Datasheet

ABclonal:Western blot - Caspase-8 Rabbit pAb (A0215)

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (A0215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Caspase-8 Rabbit pAb (A0215)

Western blot analysis of extracts of Jurkat cells, using Caspase-8 antibody (A0215) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase-8 antibody (A0215) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-8 antibody (A0215) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 antibody (A0215) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-8 Rabbit pAb(A0215)
Review
ABclonal: review for Caspase-8 Rabbit pAb(A0215)

Overview

Product nameCaspase-8 Rabbit pAb
Catalog No.A0215
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human Caspase-8 (NP_001073593.1).
SequenceMDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD
Gene ID841
Swiss protQ14790
SynonymsALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; Caspase 8; CASP8; Caspase-8; caspase-8
Calculated MW25-32kDa/45- 61kDa
Observed MW57KDa/18KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC2C12, Jurkat
Cellular locationCytoplasm
Customer validation

(Eoma cell, N9)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Ctenopharyngodon idellus, Bos taurus)

ABclonal:Western blot - Caspase-8 Rabbit pAb (A0215) }

Western blot - Caspase-8 Rabbit pAb (A0215)

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (A0215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Caspase-8 Rabbit pAb (A0215) }

Western blot - Caspase-8 Rabbit pAb (A0215)

Western blot analysis of extracts of Jurkat cells, using Caspase-8 antibody (A0215) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215) }

Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase-8 antibody (A0215) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215) }

Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-8 antibody (A0215) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Caspase-8 Rabbit pAb (A0215) }

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 antibody (A0215) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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