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Caspase-8 Rabbit pAb (A0215)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (A0215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of extracts of Jurkat cells, using Caspase-8 antibody (A0215) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat kidney using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Review

Overview

Product name	Caspase-8 Rabbit pAb
Catalog No.	A0215
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 376-479 of human Caspase-8 (NP_203519.1).
Sequence	EEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD
Gene ID	841
Swiss prot	Q14790
Synonyms	ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; Caspase 8; CASP8; Caspase-8; caspase-8
Calculated MW	25-32kDa/45- 61kDa
Observed MW	57KDa/18KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	C2C12, Jurkat
Cellular location	Cytoplasm
Customer validation	(Eoma cell, N9) WB(Mus musculus, Bos taurus, Homo sapiens, Rattus norvegicus, Ctenopharyngodon idellus, Sus scrofa) IHC(Rattus norvegicus)

Research Area

Caspase-8 Rabbit pAb images

Western blot - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of HepG2 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of PC-12 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

EMBO Molecular Medicine

Application:

IF:

12.137

Species:

Mus musculus

PMID:

29335338

Title:

CRTH2 promotes endoplasmic reticulum stress‐induced cardiomyocyte apoptosis through m‐calpain

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

5.64

Species:

Bos taurus

PMID:

29670605

Title:

Antiviral Immunotoxin Against Bovine herpesvirus-1: Targeted Inhibition of Viral Replication and Apoptosis of Infected Cell

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Investigational new drugs

Application:

IF:

3.85

Species:

Homo sapiens,Mus musculus

PMID:

30924024

Title:

Emodin induced necroptosis in the glioma cell line U251 via the TNF-α/RIP1/RIP3 pathway.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

31115582

Title:

Cytokeratin 18 regulates the transcription and alternative splicing of apoptotic‑related genes and pathways in HeLa cells.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

3.905

Species:

Mus musculus

PMID:

31202710

Title:

The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Carcinogenesis

Application:

IF:

4.944

Species:

Homo sapiens

PMID:

31306481

Title:

Histone Methyltransferase SETDB1 Promotes Colorectal Cancer Proliferation through the STAT1-CCND1/CDK6 Axis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.376

Species:

Homo sapiens,Mus musculus

PMID:

31560953

Title:

GL-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.469

Species:

Rattus norvegicus

PMID:

31221990

Title:

Microglia-derived TNF-α mediates endothelial necroptosis aggravating blood brain-barrier disruption after ischemic stroke.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Redox Biology

Application:

IF:

11.799

Species:

Mus musculus

PMID:

31926620

Title:

Triggering apoptosis by oroxylin A through caspase-8 activation and p62/SQSTM1 proteolysis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

The International Journal of Biochemistry & Cell Biology

Application:

IF:

5.085

Species:

Homo sapiens

PMID:

32097728

Title:

Downregulation of METTL14 increases apoptosis and autophagy induced by cisplatin in pancreatic cancer cells

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Molecular Neurodegeneration

Application:

IF:

14.195

Species:

Mus musculus

PMID:

32295623

Title:

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.81

Species:

Mus musculus

PMID:

29441017

Title:

15, 16-Dihydrotanshinone I Inhibits Hemangiomas through Inducing Pro-apoptotic and Anti-angiogenic Mechanisms in Vitro and in Vivo.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Homo sapiens

PMID:

31569691

Title:

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Microb Pathog

Application:

IF:

3.738

Species:

Homo sapiens

PMID:

32525021

Title:

Giardia duodenalis induces extrinsic pathway of apoptosis in intestinal epithelial cells through activation of TNFR1 and K63 de-ubiquitination of RIP1 in vitro

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Saudi Pharm J

Application:

IF:

4.33

Species:

Rattus norvegicus

PMID:

33424243

Title:

Fraxetin inhibits interleukin-1β-induced apoptosis, inflammation, and matrix degradation in chondrocytes and protects rat cartilage in vivo

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Ocul Surf

Application:

IF:

5.033

Species:

Mus musculus

PMID:

32735949

Title:

NLRP12-and NLRC4-mediated corneal epithelial pyroptosis is driven by GSDMD cleavage accompanied by IL-33 processing in dry eye

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Pharmacol Sin

Application:

IF:

6.15

Species:

Homo sapiens

PMID:

32747719

Title:

LW-213 induces cell apoptosis in human cutaneous T-cell lymphomas by activating PERK–eIF2α–ATF4–CHOP axis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Food Chem Toxicol

Application:

IF:

6.023

Species:

Homo sapiens

PMID:

32619556

Title:

Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.396

Species:

Homo sapiens

PMID:

33185634

Title:

Polycyclic polyprenylated acylphloroglucinols from Garcinia xanthochymus fruits exhibit antitumor effects by inhibition of STAT3 signaling pathway

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

33441536

Title:

FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

10.588

Species:

Ctenopharyngodon idellus

PMID:

32768820

Title:

Chlorpyrifos induces the apoptosis and necroptosis of L8824 cells through the ROS/PTEN/PI3K/AKT axis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.16

Species:

Homo sapiens

PMID:

33833498

Title:

Chlorogenic Acid Inhibits Human Glioma U373 Cell Progression via Regulating the SRC/MAPKs Signal Pathway: Based on Network Pharmacology Analysis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

10.588

Species:

Sus scrofa

PMID:

34325292

Title:

Cadmium exposure triggers Oxidative stress, Necroptosis, Th1/Th2 imbalance and promotes Inflammation through the TNF-α/NF-κB pathway in swine small intestine

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Stem Cells Dev

Application:

WB, IHC

IF:

3.272

Species:

Rattus norvegicus

PMID:

34486384

Title:

Ultrasound-Guided Transplantation of Mesenchymal Stem Cells Improves Adriamycin Nephropathy in Rats through the RIPK3/MLKL and TLR-4/NF-κB signaling

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Histochem

Application:

IF:

2.479

Species:

Rattus norvegicus

PMID:

34517258

Title:

Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Bioengineered

Application:

IF:

3.269

Species:

Mus musculus

PMID:

34898368

Title:

Cell Membrane Breakage and Triggering T Cell Infiltration are Involved in Human Telomerase Reverse Transcriptase (hTERT) Promoter-driven Novel Peptide KK-64 for liver cancer gene therapy

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Pharm Sin B

Application:

IF:

11.413

Species:

Mus musculus

PMID:

34729308

Title:

Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: Dynamic analysis on cytotoxic effects of LW-218

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Pharmaceutics

Application:

IF:

6.32

Species:

Homo sapiens

PMID:

35631550

Title:

The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death