Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Caspase-3 Rabbit pAb (A0214)

Reviews (3) Publications (76) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of various cell lines, using Caspase 3 antibody (A0214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of NIH/3T3 cells using Caspase 3 antibody (A0214) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of PC-12 cells using Caspase 3 antibody (A0214) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)

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Overview

Product name Caspase-3 Rabbit pAb
Catalog No. A0214
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
Sequence FHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID 836
Swiss prot P42574
Synonyms CPP32; SCA-1; CPP32B; Caspase-3
Calculated MW 32kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:300
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, 293T
Cellular location Cytoplasm
Customer validation

WB (Eoma cell, U2OS,Saos-2Mus musculus, Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Gallus gallus, Apocynum venetum leaf, African green monkey, gga, Bos taurus, Ctenopharyngodon idellus, Apostichopus japonicus, Dianella ensifolia, Eriocheir sinensis, Anser cygnoides)

IHC (Eoma cellMus musculus, Homo sapiens, Rattus norvegicus, Mus musculus)

IF (Mus musculus, Rattus norvegicus, Sus scrofa, Homo sapiens)

RT-PCR (Ctenopharyngodon idellus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Caspase-3 Rabbit pAb images

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)}

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of various cell lines, using Caspase 3 antibody (A0214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214)}

Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of NIH/3T3 cells using Caspase 3 antibody (A0214) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214)}

Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of PC-12 cells using Caspase 3 antibody (A0214) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on CASP3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CASP3. (Distance between topics and target gene indicate popularity.) CASP3

* Data provided by citexs.com, for reference only.

Publishing research using A0214? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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