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Caspase-3 Rabbit pAb (A0214)

Reviews (3)Publications (15) Datasheet

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)
ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)

Overview

Product nameCaspase-3 Rabbit pAb
Catalog No.A0214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human Caspase-3 (NP_004337.2).
SequenceMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTA
Gene ID836
Swiss protP42574
SynonymsCPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3
Calculated MW31kDa
Observed MW15kDa/35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, HeLa, 293T, NIH/3T3, mouse lung, mouse liver
Cellular locationCytoplasm
Customer validation

(Eoma cell, U2OS,Saos-2)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus)

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214) }

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (A0214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s
ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214) }

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of Jurkat cells, using Caspase-3 antibody (A0214) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214) }

Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of L929 cells using Caspase-3 Rabbit pAb (A0214) at dilution of 1:100. Blue: DAPI for nuclear staining.

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