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Cardiac troponin T (TNNT2) Rabbit pAb (A1126)

Publication (1) Datasheet

ABclonal:Western blot - Cardiac troponin T (TNNT2) Rabbit pAb (A1126)

Western blot analysis of extracts of various cell lines, using Cardiac troponin T (Cardiac troponin T (TNNT2)) antibody (A1126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCardiac troponin T (TNNT2) Rabbit pAb
Catalog No.A1126
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Cardiac troponin T (Cardiac troponin T (TNNT2)) (NP_001001431.1).
SequenceMSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEEL
Gene ID7139
Swiss protP45379
SynonymsTNNT2; CMD1D; CMH2; CMPD2; LVNC6; RCM3; TnTC; cTnT; troponin T2
Calculated MW30kDa/31kDa/34kDa/35kDa
Observed MW45kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesmouse heart, rat heart
Cellular location
Customer validation

WB(Gallus gallus)

ABclonal:Western blot - Cardiac troponin T (TNNT2) Rabbit pAb (A1126) }

Western blot - Cardiac troponin T (TNNT2) Rabbit pAb (A1126)

Western blot analysis of extracts of various cell lines, using Cardiac troponin T (Cardiac troponin T (TNNT2)) antibody (A1126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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