Products > Catalog Antibodies > Polyclonal Antibodies

CYP2B6 Rabbit pAb (A1463)

Publications (2) Datasheet

ABclonal:Western blot - CYP2B6 Polyclonal Antibody (A1463)

Western blot analysis of extracts of various cell lines, using CYP2B6 antibody (A1463) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded human colon using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded human breast cancer using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded mouse lung using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).

Overview

Product nameCYP2B6 Rabbit pAb
Catalog No.A1463
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1).
SequenceSKGALMDPTFLFQSITANIICSIVFGKRFHYQDQEFLKMLNLFYQTFSLISSVFGQLFELFSGFLKYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPKDLIDTYLLHMEKEKSNAHSEFSHQNLNLNTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYREIEQVIGPHRPPEL
Gene ID1555
Swiss protP20813
SynonymsCYP2B6; CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450
Calculated MW33kDa/56kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460, HT-29, Mouse liver
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - CYP2B6 Polyclonal Antibody (A1463) }

Western blot - CYP2B6 Polyclonal Antibody (A1463)

Western blot analysis of extracts of various cell lines, using CYP2B6 antibody (A1463) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463) }

Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded human colon using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463) }

Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded human breast cancer using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463) }

Immunohistochemistry - CYP2B6 Polyclonal Antibody (A1463)

Immunohistochemistry of paraffin-embedded mouse lung using CYP2B6 antibody (A1463) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A1463 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1463? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order