Products > Catalog Antibodies > Polyclonal Antibodies

CYLD Rabbit pAb (A3821)

Publications (2) Datasheet

ABclonal:Western blot - CYLD Rabbit pAb (A3821)
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)

Overview

Product nameCYLD Rabbit pAb
Catalog No.A3821
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 654-953 of human CYLD (NP_001035877.1).
SequenceTKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQVHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
Gene ID1540
Swiss protQ9NQC7
SynonymsCYLD; BRSS; CDMT; CYLD1; CYLDI; EAC; MFT; MFT1; SBS; TEM; USPL2
Calculated MW107kDa
Observed MW107KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse brain, Rat testis
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, perinuclear region, spindle
Customer validation

WB(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - CYLD Rabbit pAb (A3821) }

Western blot - CYLD Rabbit pAb (A3821)

Western blot analysis of extracts of various cell lines, using CYLD antibody (A3821) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821) }

Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded rat brain using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821) }

Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded rat spleen using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821) }

Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded human lung cancer using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821) }

Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded human esophageal cancer using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821) }

Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded mouse spleen using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

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