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CYLD Rabbit pAb (A3821)

Western blot analysis of extracts of various cell lines, using CYLD antibody (A3821) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CYLD Rabbit pAb (A3821)

Immunohistochemistry of paraffin-embedded rat brain using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CYLD Rabbit pAb (A3821) at dilution of 1:100 (40x lens).

Overview

Product name	CYLD Rabbit pAb
Catalog No.	A3821
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 654-953 of human CYLD (NP_001035877.1).
Sequence	TKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQVHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
Gene ID	1540
Swiss prot	Q9NQC7
Synonyms	CYLD; BRSS; CDMT; CYLD1; CYLDI; EAC; MFT; MFT1; SBS; TEM; USPL2
Calculated MW	107kDa
Observed MW	107KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry
Positive samples	293T, Mouse brain, Rat testis
Cellular location	Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, perinuclear region, spindle
Customer validation	WB(Rattus norvegicus, Homo sapiens)