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CXCR4 Rabbit pAb (A12534)

Publications (3) Datasheet

ABclonal:Western blot - CXCR4 Rabbit pAb (A12534)

Western blot analysis of extracts of THP-1 cells, using CXCR4 Antibody (A12534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded human prostate using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded rat lung using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded rat brain using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded mouse stomach using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

Overview

Product nameCXCR4 Rabbit pAb
Catalog No.A12534
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-42 of human CXCR4 (NP_001008540.1).
SequenceMSIPLPLLQIYTSDNYTEEMGSGDYDSMKEPCFREENANFNK
Gene ID7852
Swiss protP61073
SynonymsCD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS; CXCR4
Calculated MW39kDa/40kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1
Cellular locationCell junction, Cell membrane, Early endosome, Late endosome, Lysosome, Multi-pass membrane protein
Customer validation

WB(Mus musculus, Rattus norvegicus)

IHC(Homo sapiens)

ABclonal:Western blot - CXCR4 Rabbit pAb (A12534) }

Western blot - CXCR4 Rabbit pAb (A12534)

Western blot analysis of extracts of THP-1 cells, using CXCR4 Antibody (A12534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534) }

Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded human prostate using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534) }

Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded rat lung using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534) }

Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded rat brain using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Rabbit pAb (A12534) }

Immunohistochemistry - CXCR4 Rabbit pAb (A12534)

Immunohistochemistry of paraffin-embedded mouse stomach using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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