Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CXCR4 Rabbit pAb (A1303)

Review (1) Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - CXCR4 Rabbit pAb (A1303)

Western blot analysis of extracts of various cell lines, using CXCR4 antibody (A1303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for CXCR4 Rabbit pAb(A1303)

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Overview

Product name CXCR4 Rabbit pAb
Catalog No. A1303
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CXCR4 (NP_003458.1).
Sequence MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVA
Gene ID 7852
Swiss prot P61073
Synonyms FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; WHIMS1; D2S201E; CXCR4
Calculated MW 40kDa
Observed MW 36kDa/70kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples SH-SY5Y, K-562, HeLa, SW480, B-cell, Mouse thymus
Cellular location Cell junction, Cell membrane, Early endosome, Late endosome, Lysosome, Multi-pass membrane protein
Customer validation

(umbilical endothelial cells)

WB (Homo sapiens, Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CXCR4 Rabbit pAb images

ABclonal:Western blot - CXCR4 Rabbit pAb (A1303)}

Western blot - CXCR4 Rabbit pAb (A1303)

Western blot analysis of extracts of various cell lines, using CXCR4 antibody (A1303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on CXCR4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CXCR4. (Distance between topics and target gene indicate popularity.) CXCR4

* Data provided by citexs.com, for reference only.

Publishing research using A1303? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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