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CXCR3 Rabbit pAb (A2939)

Publications (4) Datasheet

ABclonal:Western blot - CXCR3 Rabbit pAb (A2939)

Western blot analysis of extracts of various cell lines, using CXCR3 antibody (A2939) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - CXCR3 Rabbit pAb (A2939)

Western blot analysis of extracts of various cell lines, using CXCR3 antibody (A2939) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded human liver cancer using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded mouse heart using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).

Overview

Product nameCXCR3 Rabbit pAb
Catalog No.A2939
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 289-368 of human CXCR3 (NP_001495.1).
SequenceEmail for sequence
Gene ID2833
Swiss protP49682
SynonymsCXCR3; CD182; CD183; CKR-L2; CMKAR3; GPR9; IP10-R; Mig-R; MigR
Calculated MW28kDa/40kDa/45kDa
Observed MW44kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, K-562, HepG2, SGC996, SW620
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

IHC(Mus musculus, Homo sapiens)

WB(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - CXCR3 Rabbit pAb (A2939) }

Western blot - CXCR3 Rabbit pAb (A2939)

Western blot analysis of extracts of various cell lines, using CXCR3 antibody (A2939) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - CXCR3 Rabbit pAb (A2939) }

Western blot - CXCR3 Rabbit pAb (A2939)

Western blot analysis of extracts of various cell lines, using CXCR3 antibody (A2939) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939) }

Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded human liver cancer using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939) }

Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR3 Rabbit pAb (A2939) }

Immunohistochemistry - CXCR3 Rabbit pAb (A2939)

Immunohistochemistry of paraffin-embedded mouse heart using CXCR3 Antibody (A2939) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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