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CTSK Rabbit pAb (A1782)

Publications (5) Datasheet

ABclonal:Western blot - CTSK Polyclonal Antibody (A1782)

Western blot analysis of extracts of mouse liver, using CTSK antibody (A1782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded mouse brain using CTSK antibody (A1782) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded rat brain using CTSK antibody (A1782) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded mouse lung using CTSK antibody (A1782) at dilution of 1:100 (40x lens).

Overview

Product nameCTSK Rabbit pAb
Catalog No.A1782
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 115-329 of human CTSK (NP_000387.1).
SequenceAPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGCGGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFPKM
Gene ID1513
Swiss protP43235
SynonymsCTSK; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
Calculated MW36kDa
Observed MW41kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse liver
Cellular locationLysosome
Customer validation

IHC(Mus musculus)

WB(Rattus norvegicus, Meretrix meretrix)

IF(Mus musculus)

ABclonal:Western blot - CTSK Polyclonal Antibody (A1782) }

Western blot - CTSK Polyclonal Antibody (A1782)

Western blot analysis of extracts of mouse liver, using CTSK antibody (A1782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782) }

Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded mouse brain using CTSK antibody (A1782) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782) }

Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded rat brain using CTSK antibody (A1782) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CTSK Polyclonal Antibody (A1782) }

Immunohistochemistry - CTSK Polyclonal Antibody (A1782)

Immunohistochemistry of paraffin-embedded mouse lung using CTSK antibody (A1782) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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