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CTR1/SLC31A1 Rabbit mAb (A0773)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - CTR1/SLC31A1 Rabbit mAb (A0773)

Western blot analysis of extracts of various cell lines, using CTR1/SLC31A1 antibody (A0773) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameCTR1/SLC31A1 Rabbit mAb
Catalog No.A0773
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CTR1/SLC31A1 (O15431).
SequenceMDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAGEMAGAFVAVFLLAMFYEGLKIARESLLRKSQVS
Gene ID1317
Swiss protO15431
SynonymsSLC31A1; COPT1; CTR1; solute carrier family 31 member 1
Calculated MW26-34kDa
Observed MW32KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, HepG2, A-549, Mouse liver, Mouse kidney, Rat liver
Cellular locationCell membrane, Multi-pass membrane protein
    ABclonal:Western blot - CTR1/SLC31A1 Rabbit mAb (A0773)}

    Western blot - CTR1/SLC31A1 Rabbit mAb (A0773)

    Western blot analysis of extracts of various cell lines, using CTR1/SLC31A1 antibody (A0773) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

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