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[KO Validated] α-Catenin Rabbit pAb (A5635)

Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635)

Western blot analysis of extracts of various cell lines, using α-Catenin antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635)

Western blot analysis of extracts from normal (control) and α-Catenin Rabbit pAb knockout (KO) 293T cells, using α-Catenin Rabbit pAb antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded rat kidney using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded human vermiform appendix using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded human stomach using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded mouse testis using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug α-Catenin antibody (A5635). Western blot was performed from the immunoprecipitate using α-Catenin antibody (A5635) at a dilition of 1:500.

Overview

Product name[KO Validated] α-Catenin Rabbit pAb
Catalog No.A5635
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human α-Catenin (NP_001894.2).
SequenceELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI
Gene ID1495
Swiss protP35221
SynonymsCAP102; MDPT2; Catenin alpha 1; CTNNA1
Calculated MW59kDa/100kDa/102kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesBT-474, OVCAR3, 293T, MCF7, Mouse lung, Mouse heart, Mouse brain
Cellular locationCell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton
Customer validation

WB(Toxoplasma gondii, Mus musculus, Homo sapiens)

ABclonal:Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635)

Western blot analysis of extracts of various cell lines, using α-Catenin antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Western blot - [KO Validated] α-Catenin Rabbit pAb (A5635)

Western blot analysis of extracts from normal (control) and α-Catenin Rabbit pAb knockout (KO) 293T cells, using α-Catenin Rabbit pAb antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded rat kidney using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded human vermiform appendix using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded human stomach using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Immunohistochemistry - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunohistochemistry of paraffin-embedded mouse testis using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] α-Catenin Rabbit pAb (A5635) }

Immunoprecipitation - [KO Validated] α-Catenin Rabbit pAb (A5635)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug α-Catenin antibody (A5635). Western blot was performed from the immunoprecipitate using α-Catenin antibody (A5635) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

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