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CTNNA1 Polyclonal Antibody (A5635)

Publication (1) Datasheet

ABclonal:Western blot - CTNNA1 Polyclonal Antibody (A5635)
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)
ABclonal:Immunoprecipitation - CTNNA1 Polyclonal Antibody (A5635)

Overview

Product nameCTNNA1 Polyclonal Antibody
Catalog No.A5635
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
SequenceELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI
Gene ID1495
Swiss protP35221
SynonymsCAP102; MDPT2; Catenin alpha 1; CTNNA1
Calculated MW59kDa/100kDa/102kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesBT-474, OVCAR3, 293T, MCF7, Mouse lung, Mouse heart, Mouse brain
Cellular locationCell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton
Customer validation

WB(T. gondii)

ABclonal:Western blot - CTNNA1 Polyclonal Antibody (A5635) }

Western blot - CTNNA1 Polyclonal Antibody (A5635)

Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635) }

Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)

Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635) }

Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)

Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635) }

Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)

Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635) }

Immunohistochemistry - CTNNA1 Polyclonal Antibody (A5635)

Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (A5635) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - CTNNA1 Polyclonal Antibody (A5635) }

Immunoprecipitation - CTNNA1 Polyclonal Antibody (A5635)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody (A5635). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (A5635) at a dilition of 1:500.

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