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CTCF Rabbit pAb (A1133)

Publication (1) Datasheet

ABclonal:CTCF Rabbit pAb
ABclonal:Western blot - CTCF Rabbit pAb (A1133)

Western blot analysis of extracts of various cell lines, using CTCF antibody (A1133) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - CTCF Rabbit pAb (A1133)

Western blot analysis of extracts of NIH/3T3 cells, using CTCF antibody (A1133) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133)

Immunohistochemistry of paraffin-embedded Mouse brain using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133)

Immunohistochemistry of paraffin-embedded Rat brain using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133)

Immunohistochemistry of paraffin-embedded Human colon using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133)

Immunofluorescence analysis of NIH/3T3 cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133)

Immunofluorescence analysis of PC-12 cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133)

Immunofluorescence analysis of U2OS cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CTCF Rabbit pAb (A1133)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (A1133). Western blot was performed from the immunoprecipitate using CTCF antibody (A1133) at a dilition of 1:1000.

ABclonal:Chromatin Immunoprecipitation - CTCF Rabbit pAb (A1133)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody (A1133) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameCTCF Rabbit pAb
Catalog No.A1133
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
SequenceMEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKK
Gene ID10664
Swiss protP49711
SynonymsCTCF; MRD21; CTCF
Calculated MW45kDa/82kDa
Observed MW140KDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
ChIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, SH-SY5Y, NIH/3T3
Cellular locationChromosome, Nucleus, centromere, nucleoplasm
Customer validation

.(Homo sapiens)

    ABclonal:Western blot - CTCF Rabbit pAb (A1133) }

    Western blot - CTCF Rabbit pAb (A1133)

    Western blot analysis of extracts of various cell lines, using CTCF antibody (A1133) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - CTCF Rabbit pAb (A1133) }

    Western blot - CTCF Rabbit pAb (A1133)

    Western blot analysis of extracts of NIH/3T3 cells, using CTCF antibody (A1133) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133) }

    Immunohistochemistry - CTCF Rabbit pAb (A1133)

    Immunohistochemistry of paraffin-embedded Mouse brain using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133) }

    Immunohistochemistry - CTCF Rabbit pAb (A1133)

    Immunohistochemistry of paraffin-embedded Rat brain using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - CTCF Rabbit pAb (A1133) }

    Immunohistochemistry - CTCF Rabbit pAb (A1133)

    Immunohistochemistry of paraffin-embedded Human colon using CTCF Rabbit pAb (A1133) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133) }

    Immunofluorescence - CTCF Rabbit pAb (A1133)

    Immunofluorescence analysis of NIH/3T3 cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133) }

    Immunofluorescence - CTCF Rabbit pAb (A1133)

    Immunofluorescence analysis of PC-12 cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CTCF Rabbit pAb (A1133) }

    Immunofluorescence - CTCF Rabbit pAb (A1133)

    Immunofluorescence analysis of U2OS cells using CTCF Rabbit pAb (A1133) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - CTCF Rabbit pAb (A1133) }

    Immunoprecipitation - CTCF Rabbit pAb (A1133)

    Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (A1133). Western blot was performed from the immunoprecipitate using CTCF antibody (A1133) at a dilition of 1:1000.
    ABclonal:Chromatin Immunoprecipitation - CTCF Rabbit pAb (A1133) }

    Chromatin Immunoprecipitation - CTCF Rabbit pAb (A1133)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody (A1133) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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