Products > Catalog Antibodies > Polyclonal Antibodies

CSF3 Rabbit pAb (A6178)

Datasheet

ABclonal:Western blot - CSF3 Polyclonal Antibody (A6178)

Western blot analysis of extracts of various cell lines, using CSF3 antibody (A6178) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded rat spleen using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded human colon using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded mouse spleen using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).

Overview

Product nameCSF3 Rabbit pAb
Catalog No.A6178
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human CSF3 (NP_000750.1).
SequenceATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLVSECATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
Gene ID1440
Swiss protP09919
SynonymsCSF3; C17orf33; CSF3OS; GCSF
Calculated MW18kDa/21kDa/22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHL-60, 293T, Mouse liver
Cellular locationSecreted
ABclonal:Western blot - CSF3 Polyclonal Antibody (A6178) }

Western blot - CSF3 Polyclonal Antibody (A6178)

Western blot analysis of extracts of various cell lines, using CSF3 antibody (A6178) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178) }

Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded rat spleen using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178) }

Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded human colon using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CSF3 Polyclonal Antibody (A6178) }

Immunohistochemistry - CSF3 Polyclonal Antibody (A6178)

Immunohistochemistry of paraffin-embedded mouse spleen using CSF3 antibody (A6178) at dilution of 1:100 (40x lens).

Inquire About This Product

Submit your question about A6178 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order