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CRNN Polyclonal Antibody (A8762)

Datasheet

ABclonal:Western blot - CRNN Polyclonal Antibody (A8762)

Overview

Product nameCRNN Polyclonal Antibody
Catalog No.A8762
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the 'fused gene' family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. The encoded protein contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This gene, also known as squamous epithelial heat shock protein 53, may play a role in the mucosal/epithelial immune response and epidermal differentiation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 396-495 of human CRNN (NP_057274.1).
SequenceGETVPGGQAQTGASTESGRQEWSSTHPRRCVTEGQGDRQPTVVGEEWVDDHSRETVILRLDQGNLHTSVSSAQGQDAAQSEEKRGITARELYSYLRSTKP
Gene ID49860
Swiss protQ9UBG3
SynonymsCRNN; C1orf10; DRC1; PDRC1; SEP53; cornulin
Calculated MW53kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, TE-1, 293T, HepG2, Mouse kidney
Cellular locationCytoplasm
ABclonal:Western blot - CRNN Polyclonal Antibody (A8762) }

Western blot - CRNN Polyclonal Antibody (A8762)

Western blot analysis of extracts of various cell lines, using CRNN antibody (A8762) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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