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CREB1 Rabbit pAb (A11989)

Publications (5) Datasheet

ABclonal:Western blot - CREB1 Rabbit pAb (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 antibody (A11989) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - CREB1 Rabbit pAb (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 Rabbit pAb (A11989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded rat brain using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded human lymph node using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of NIH/3T3 cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of PC-12 cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of U2OS cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CREB1 Rabbit pAb (A11989)

Immunoprecipitation analysis of 300ug extracts of LO2 cells using 3ug CREB1 antibody (A11989). Western blot was performed from the immunoprecipitate using CREB1 antibody (A11989) at a dilition of 1:1000.

Overview

Product nameCREB1 Rabbit pAb
Catalog No.A11989
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human CREB1 (NP_604391.1).
SequenceMTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
Gene ID1385
Swiss protP16220
SynonymsCREB1; CREB; CREB-1
Calculated MW25kDa/35kDa/36kDa
Observed MW37KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Jurkat, C6
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - CREB1 Rabbit pAb (A11989) }

Western blot - CREB1 Rabbit pAb (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 antibody (A11989) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - CREB1 Rabbit pAb (A11989) }

Western blot - CREB1 Rabbit pAb (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 Rabbit pAb (A11989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989) }

Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded rat brain using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989) }

Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded human lymph node using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989) }

Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody (A11989) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989) }

Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of NIH/3T3 cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989) }

Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of PC-12 cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989) }

Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of U2OS cells using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CREB1 Rabbit pAb (A11989) }

Immunoprecipitation - CREB1 Rabbit pAb (A11989)

Immunoprecipitation analysis of 300ug extracts of LO2 cells using 3ug CREB1 antibody (A11989). Western blot was performed from the immunoprecipitate using CREB1 antibody (A11989) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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