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CREB1 Polyclonal Antibody (A11989)

Publications (2) Datasheet

ABclonal:Western blot - CREB1 Polyclonal Antibody (A11989)
ABclonal:Immunofluorescence - CREB1 Polyclonal Antibody (A11989)

Overview

Product nameCREB1 Polyclonal Antibody
Catalog No.A11989
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human CREB1 (NP_604391.1).
SequenceMTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
Gene ID1385
Swiss protP16220
SynonymsCREB1; CREB; CREB-1; cAMP responsive element binding protein 1
Calculated MW25kDa/35kDa/36kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, Jurkat, LO2, Mouse brain, Rat brain
Cellular locationNucleus
Customer validation

WB(Human, mouse, rat)

ABclonal:Western blot - CREB1 Polyclonal Antibody (A11989) }

Western blot - CREB1 Polyclonal Antibody (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 antibody (A11989) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - CREB1 Polyclonal Antibody (A11989) }

Immunofluorescence - CREB1 Polyclonal Antibody (A11989)

Immunofluorescence analysis of HeLa cells using CREB1 antibody (A11989) at dilution of 1:100. Blue: DAPI for nuclear staining.

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