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CPT1B Rabbit pAb (A6796)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - CPT1B Polyclonal Antibody (A6796)

Western blot analysis of extracts of various cell lines, using CPT1B antibody (A6796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded rat liver using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded human colon using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded mouse heart using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CPT1B Polyclonal Antibody (A6796)

Immunofluorescence analysis of NIH/3T3 cells using CPT1B antibody (A6796) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CPT1B Polyclonal Antibody (A6796)

Immunofluorescence analysis of U2OS cells using CPT1B antibody (A6796) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for CPT1B Rabbit pAb(A6796)

Overview

Product nameCPT1B Rabbit pAb
Catalog No.A6796
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).
SequenceSYQVAKALADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMTRMFREGRTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCLYLVSKYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGYGVSYMIAGENTIFFHISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS
Gene ID1375
Swiss protQ92523
SynonymsCPT1B; CPT1-M; CPT1M; CPTI; CPTI-M; M-CPT1; MCCPT1; MCPT1
Calculated MW66kDa/78kDa/83kDa/87kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, B cells, Mouse kidney, Mouse lung
Cellular locationMitochondrion outer membrane, Multi-pass membrane protein
Customer validation

(Mouse brown fat cells)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - CPT1B Polyclonal Antibody (A6796) }

Western blot - CPT1B Polyclonal Antibody (A6796)

Western blot analysis of extracts of various cell lines, using CPT1B antibody (A6796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796) }

Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded rat liver using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796) }

Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded human colon using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CPT1B Polyclonal Antibody (A6796) }

Immunohistochemistry - CPT1B Polyclonal Antibody (A6796)

Immunohistochemistry of paraffin-embedded mouse heart using CPT1B antibody (A6796) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CPT1B Polyclonal Antibody (A6796) }

Immunofluorescence - CPT1B Polyclonal Antibody (A6796)

Immunofluorescence analysis of NIH/3T3 cells using CPT1B antibody (A6796) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CPT1B Polyclonal Antibody (A6796) }

Immunofluorescence - CPT1B Polyclonal Antibody (A6796)

Immunofluorescence analysis of U2OS cells using CPT1B antibody (A6796) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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