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CPT1A Rabbit pAb (A5307)

Reviews (3)Publications (23) Datasheet

ABclonal:Western blot - CPT1A Rabbit pAb (A5307)

Western blot analysis of extracts of various cell lines, using CPT1A antibody (A5307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - CPT1A Rabbit pAb (A5307)

Immunofluorescence analysis of HeLa cells using CPT1A Rabbit pAb (A5307) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CPT1A Rabbit pAb (A5307)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug CPT1A antibody (A5307). Western blot was performed from the immunoprecipitate using CPT1A antibody (A5307) at a dilition of 1:1000.

Review
ABclonal: review for CPT1A Rabbit pAb(A5307)
Review
ABclonal: review for CPT1A Rabbit pAb(A5307)
Review
ABclonal: review for CPT1A Rabbit pAb(A5307)

Overview

Product nameCPT1A Rabbit pAb
Catalog No.A5307
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A (NP_001027017.1).
SequenceGYAEDGHCKGDINPNIPYPTRLQWDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLINFHISSKFSCPETGIISQGPSSDT
Gene ID1374
Swiss protP50416
SynonymsCPT1A; CPT1; CPT1-L; L-CPT1
Calculated MW86kDa/88kDa
Observed MW88kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7, HepG2, Mouse heart, Rat heart
Cellular locationMitochondrion outer membrane, Multi-pass membrane protein
Customer validation

(Human lung cancer cells, Human bone marrow cancer cell)

WB(Homo sapiens, Mus musculus, Sus scrofa, Gallus gallus, Rattus norvegicus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - CPT1A Rabbit pAb (A5307) }

Western blot - CPT1A Rabbit pAb (A5307)

Western blot analysis of extracts of various cell lines, using CPT1A antibody (A5307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - CPT1A Rabbit pAb (A5307) }

Immunofluorescence - CPT1A Rabbit pAb (A5307)

Immunofluorescence analysis of HeLa cells using CPT1A Rabbit pAb (A5307) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CPT1A Rabbit pAb (A5307) }

Immunoprecipitation - CPT1A Rabbit pAb (A5307)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug CPT1A antibody (A5307). Western blot was performed from the immunoprecipitate using CPT1A antibody (A5307) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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