Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

COX7A2 Rabbit pAb (A8406)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - COX7A2 Rabbit pAb (A8406)

Western blot analysis of extracts of HeLa cells, using COX7A2 antibody (A8406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Overview

Product name COX7A2 Rabbit pAb
Catalog No. A8406
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 33-115 of human COX7A2 (NP_001856.2).
Sequence MLRNLLALRQIGQRTISTASRRHFKNKVPEKQKLFQEDDEIPLYLKGGVADALLYRATMILTVGGTAYAIYELAVASFPKKQE
Gene ID 1347
Swiss prot P14406
Synonyms VIIAL; COX7AL; COX7AL1; COXVIIAL; COXVIIa-L; COX7A2
Calculated MW 9kDa
Observed MW 11kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Mitochondrion inner membrane
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

COX7A2 Rabbit pAb images

ABclonal:Western blot - COX7A2 Rabbit pAb (A8406)}

Western blot - COX7A2 Rabbit pAb (A8406)

Western blot analysis of extracts of HeLa cells, using COX7A2 antibody (A8406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on COX7A2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to COX7A2. (Distance between topics and target gene indicate popularity.) COX7A2

* Data provided by citexs.com, for reference only.

Publishing research using A8406? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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