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COX7A2L Polyclonal Antibody (A8298)

Datasheet

ABclonal:Western blot - COX7A2L Polyclonal Antibody (A8298)

Overview

Product nameCOX7A2L Polyclonal Antibody
Catalog No.A8298
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human COX7A2L (NP_004709.2).
SequenceMYYKFSGFTQKLAGAWASEAYSPQGLKPVVSTEAPPIIFATPTKLTSDSTVYDYAGKNKVPELQKFFQKADGVPVYLKRGLPDQMLYRTTMALTVGGTIYCLIALYMASQPKNK
Gene ID9167
Swiss protO14548
SynonymsCOX7A2L; COX7AR; COX7RP; EB1; SIG81
Calculated MW12kDa
Observed MW13kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMCF7, A-549, HeLa, Mouse brain
Cellular locationMitochondrion inner membrane
ABclonal:Western blot - COX7A2L Polyclonal Antibody (A8298) }

Western blot - COX7A2L Polyclonal Antibody (A8298)

Western blot analysis of extracts of various cell lines, using COX7A2L antibody (A8298) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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