Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

COX2/PTGS2 Rabbit pAb (A1253)

Reviews (3) Publications (119) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - COX2/PTGS2 Rabbit pAb (A1253)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit pAb (A1253) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded human breast cancer using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded human colon using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)
Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)
Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)

You may also interested in:

Overview

Product name COX2/PTGS2 Rabbit pAb
Catalog No. A1253
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of COX2/PTGS2 (NP_000954.1).
Sequence PLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL
Gene ID 5743
Swiss prot P35354
Synonyms COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2/PTGS2
Calculated MW 69kDa
Observed MW 69kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse uterus, Mouse lung
Cellular location Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Customer validation

WB (Mouse embryonal fibroblast cell, RAW264.7,Mouse kidneyMus musculus, Rattus norvegicus, Mus musculus, Schisandra chinensis, Homo sapiens, Trifolium pratense, Gallus gallus, Boswellia carterii, Sus scrofa, Carassius gibelio, Anser cygnoides)

IHC (RAW264.7,Mouse kidneyMus musculus, Rattus norvegicus, Mus musculus, Homo sapiens, mus musculus)

IF (Mus musculus, Homo sapiens, Rattus norvegicus)

Histological staining (Mus musculus)

WB IHC (Homo sapiens)

Bos taurus (WB)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

COX2/PTGS2 Rabbit pAb images

ABclonal:Western blot - COX2/PTGS2 Rabbit pAb (A1253)}

Western blot - COX2/PTGS2 Rabbit pAb (A1253)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit pAb (A1253) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)}

Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded human breast cancer using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)}

Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded human colon using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Submit an review for A1253

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1253 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on PTGS2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PTGS2. (Distance between topics and target gene indicate popularity.) PTGS2

* Data provided by citexs.com, for reference only.

Publishing research using A1253? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order