Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

COL2A1 Rabbit pAb (A1560)

Review (1) Publications (24) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - COL2A1 Rabbit pAb (A1560)

Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Rabbit pAb (A1560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - COL2A1 Rabbit pAb (A1560)

Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit pAb (A1560) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for COL2A1 Rabbit pAb(A1560)

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Overview

Product name COL2A1 Rabbit pAb
Catalog No. A1560
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1220-1282 of human COL2A1 (NP_001835.3).
Sequence MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART
Gene ID 1280
Swiss prot P02458
Synonyms AOM; ANFH; SEDC; STL1; COL11A3; COL2A1
Calculated MW 142kDa
Observed MW 141kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse skeletal muscle
Cellular location Secreted, extracellular matrix, extracellular space
Customer validation

IHC (Mouse intervertebral discRattus norvegicus, Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

WB (Rattus norvegicus, Gallus gallus, Homo sapiens, Mus musculus)

WB IHC (Mus musculus)

IF (Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

PCR (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

COL2A1 Rabbit pAb images

ABclonal:Western blot - COL2A1 Rabbit pAb (A1560)}

Western blot - COL2A1 Rabbit pAb (A1560)

Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Rabbit pAb (A1560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - COL2A1 Rabbit pAb (A1560)}

Immunofluorescence - COL2A1 Rabbit pAb (A1560)

Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit pAb (A1560) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on COL2A1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to COL2A1. (Distance between topics and target gene indicate popularity.) COL2A1

* Data provided by citexs.com, for reference only.

Publishing research using A1560? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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