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CNP Polyclonal Antibody (A1018)

Publication (1) Datasheet

ABclonal:Western blot - CNP antibody (A1018)
ABclonal:Immunohistochemistry - CNP antibody (A1018)
ABclonal:Immunohistochemistry - CNP antibody (A1018)

Overview

Product nameCNP Polyclonal Antibody
Catalog No.A1018
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 152-421 of human CNP (NP_149124.3).
SequenceTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII
Gene ID1267
Swiss protP09543
SynonymsCNP1
Calculated MW45kDa/47kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Rat brain, 293T, Mouse brain
Cellular locationLipid-anchor, Melanosome, Membrane
Customer validation

WB(Human)

ABclonal:Western blot - CNP antibody (A1018)}

Western blot - CNP antibody (A1018)

Western blot analysis of extracts of various cell lines, using CNP antibody (A1018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 15s.
ABclonal:Immunohistochemistry - CNP antibody (A1018)}

Immunohistochemistry - CNP antibody (A1018)

Immunohistochemistry of paraffin-embedded rat brain using CNP antibody (A1018) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CNP antibody (A1018)}

Immunohistochemistry - CNP antibody (A1018)

Immunohistochemistry of paraffin-embedded mouse spinal cord using CNP antibody (A1018) at dilution of 1:100 (40x lens).

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