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CLDN11 Rabbit pAb (A12478)

Datasheet

ABclonal:Western blot - CLDN11 Polyclonal Antibody (A12478)

Western blot analysis of extracts of various cell lines, using CLDN11 antibody (A12478) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product nameCLDN11 Rabbit pAb
Catalog No.A12478
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a major component of central nervous system (CNS) myelin and plays an important role in regulating proliferation and migration of oligodendrocytes. Mouse studies showed that the gene deficiency results in deafness and loss of the Sertoli cell epithelial phenotype in the testis. This protein is a tight junction protein at the human blood-testis barrier (BTB), and the BTB disruption is related to a dysfunction of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CLDN11 (NP_005593.2).
SequenceMVATCLQVVGFVTSFVGWIGVIVTTSTNDWVVTCGYTIPTCRKLDELGSKGLWADCVMATGLYHCKPLVDILILPGYVQACRALMIAASVLGLPAILLLL
Gene ID5010
Swiss protO75508
SynonymsCLDN11; OSP; OTM
Calculated MW21kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460, DU145, SW480, Mouse kidney, Mouse lung, Rat brain, Rat kidney, Rat lung
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, tight junction
ABclonal:Western blot - CLDN11 Polyclonal Antibody (A12478) }

Western blot - CLDN11 Polyclonal Antibody (A12478)

Western blot analysis of extracts of various cell lines, using CLDN11 antibody (A12478) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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