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CHRM3 Rabbit pAb (A1602)

Publications (3) Datasheet

ABclonal:Western blot - CHRM3 Polyclonal Antibody (A1602)

Western blot analysis of extracts of various cell lines, using CHRM3 antibody (A1602) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameCHRM3 Rabbit pAb
Catalog No.A1602
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 390-475 of human CHRM3 (NP_000731.1).
SequenceQVPEEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQI
Gene ID1131
Swiss protP20309
SynonymsCHRM3; EGBRS; HM3; PBS
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSH-SY5Y, Mouse brain, Mouse heart, Rat heart
Cellular locationBasolateral cell membrane, Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Customer validation

IHC(Rattus norvegicus)

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - CHRM3 Polyclonal Antibody (A1602) }

Western blot - CHRM3 Polyclonal Antibody (A1602)

Western blot analysis of extracts of various cell lines, using CHRM3 antibody (A1602) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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