Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CER1 Rabbit pAb (A7354)

Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CER1 Rabbit pAb (A7354)

Western blot analysis of extracts of various cell lines, using CER1 Antibody (A7354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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    Overview

    Product nameCER1 Rabbit pAb
    Catalog No.A7354
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-267 of human CER1 (NP_005445.1).
    SequenceTRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVATSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKVVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA
    Gene ID9350
    Swiss protO95813
    SynonymsDAND4
    Calculated MW30kDa
    Observed MW30kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:2000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    Application keyWestern blotting    
    Positive samplesMouse liver, Rat liver
    Cellular locationSecreted

    CER1 Rabbit pAb images

    ABclonal:Western blot - CER1 Rabbit pAb (A7354)}

    Western blot - CER1 Rabbit pAb (A7354)

    Western blot analysis of extracts of various cell lines, using CER1 Antibody (A7354) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on CER1. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to CER1. (Distance between topics and target gene indicate popularity.) CER1

    * Data provided by citexs.com, for reference only.

    Publishing research using A7354? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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