Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDKN2A/p16INK4a Mouse mAb (A20371)

PathoQIHC Pathology

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Immunohistochemistry - CDKN2A/p16INK4a Mouse mAb (A20371)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer using CDKN2A/p16INK4a Mouse mAb (A20371) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name CDKN2A/p16INK4a Mouse mAb
Catalog No. A20371
Host species Mouse
Purification method Cell culture supernatant concentrate
Isotype IgG2a, kappa
CloneNo. AMC0530

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Immunogen information

Immunogen Recombinant protein of human CDKN2A/p16INK4a.
Sequence Email for sequence
Gene ID 1029
Swiss prot P42771Q8N726
Synonyms ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A; CDKN2A/p16INK4a
Calculated MW 8kDa/11kDa/12kDa/13kDa/16kDa/17kDa
Observed MW 17kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Tris Buffer, pH7.3-7.7, with 1% BSA and <0.1% Sodium azide.
Application key Western blotting    Immunohistochemistry    
Positive samples
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

CDKN2A/p16INK4a Mouse mAb images

ABclonal:Immunohistochemistry - CDKN2A/p16INK4a Mouse mAb (A20371)}

Immunohistochemistry - CDKN2A/p16INK4a Mouse mAb (A20371)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer using CDKN2A/p16INK4a Mouse mAb (A20371) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDKN2A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDKN2A. (Distance between topics and target gene indicate popularity.) CDKN2A

* Data provided by citexs.com, for reference only.

Publishing research using A20371? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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