Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDKN1B/p27KIP1 Rabbit mAb (A19095)

PathoQIHC Pathology

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (A19095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human brain using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human placenta using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human spleen using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human thyroid using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human tonsil using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded rat ovary using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name CDKN1B/p27KIP1 Rabbit mAb
Catalog No. A19095
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0455

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 99-198 of human CDKN1B/p27KIP1 (P46527).
Sequence CKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT
Gene ID 1027
Swiss prot P46527
Synonyms KIP1; MEN4; CDKN4; MEN1B; P27KIP1; CDKN1B/p27KIP1
Calculated MW 22kDa
Observed MW 27kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanRat
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2 cells, 293T cells, C6 cells, HeLa cells, Mouse lung, Rat lung
Cellular location Cytoplasm, Endosome, Nucleus
Customer validation

WB (Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CDKN1B/p27KIP1 Rabbit mAb images

ABclonal:Western blot - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Western blot - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (A19095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human brain using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human placenta using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human spleen using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human thyroid using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded human tonsil using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)}

Immunohistochemistry - CDKN1B/p27KIP1 Rabbit mAb (A19095)

Immunohistochemistry analysis of paraffin-embedded rat ovary using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDKN1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDKN1B. (Distance between topics and target gene indicate popularity.) CDKN1B

* Data provided by citexs.com, for reference only.

Publishing research using A19095? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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