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CDKN1A/p21CIP1 Rabbit pAb (A1483)

Reviews (3)Publications (19) Datasheet

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A1483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of extracts of 293T cells, using CDKN1A/p21CIP1 antibody (A1483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)

Overview

Product nameCDKN1A/p21CIP1 Rabbit pAb
Catalog No.A1483
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 43-142 of human CDKN1A/p21CIP1 (NP_000380.1).
SequenceQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKR
Gene ID1026
Swiss protP38936
SynonymsCAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKN1A
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse heart, Rat spinal cord
Cellular locationCytoplasm, Nucleus
Customer validation

(rat cells, P19, P21)

WB(Homo sapiens, Mus musculus, Sanghuangporus vaninii)

ChIP(Rattus norvegicus)

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483) }

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A1483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483) }

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of extracts of 293T cells, using CDKN1A/p21CIP1 antibody (A1483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483) }

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483) }

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483) }

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 antibody (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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