Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDKN1A/p21CIP1 Rabbit mAb (A11877)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit mAb (A11877)

Western blot analysis of various lysates using CDKN1A/p21CIP1 Rabbit mAb (A11877) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name CDKN1A/p21CIP1 Rabbit mAb
Catalog No. A11877
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human CDKN1A/p21CIP1
Sequence Email for sequence
Gene ID 1026
Swiss prot P38936
Synonyms P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; CDKN1A/p21CIP1
Calculated MW 18kDa
Observed MW 18kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, U-87MG
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

CDKN1A/p21CIP1 Rabbit mAb images

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit mAb (A11877)}

Western blot - CDKN1A/p21CIP1 Rabbit mAb (A11877)

Western blot analysis of various lysates using CDKN1A/p21CIP1 Rabbit mAb (A11877) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDKN1A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDKN1A. (Distance between topics and target gene indicate popularity.) CDKN1A

* Data provided by citexs.com, for reference only.

Publishing research using A11877? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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