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CDK4 Rabbit pAb (A0366)

Reviews (3)Publications (14) Datasheet

ABclonal:Western blot - CDK4 Rabbit pAb (A0366)

Western blot analysis of extracts of various cell lines, using CDK4 antibody (A0366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - CDK4 Rabbit pAb (A0366)

Western blot analysis of extracts of various cell lines, using CDK4 antibody (A0366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - CDK4 Rabbit pAb (A0366)

Immunohistochemistry of paraffin-embedded human colon using CDK4 antibody (A0366) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CDK4 Rabbit pAb (A0366)

Immunofluorescence analysis of A-549 cells using CDK4 antibody (A0366). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CDK4 Rabbit pAb (A0366)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody (A0366). Western blot was performed from the immunoprecipitate using CDK4 antibody (A0366) at a dilition of 1:1000.

Review
ABclonal: review for CDK4 Rabbit pAb(A0366)
Review
ABclonal: review for CDK4 Rabbit pAb(A0366)
Review
ABclonal: review for CDK4 Rabbit pAb(A0366)

Overview

Product nameCDK4 Rabbit pAb
Catalog No.A0366
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CDK4 (NP_000066.1).
SequenceMATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE
Gene ID1019
Swiss protP11802
SynonymsCDK4; CMM3; PSK-J3
Calculated MW20kDa/33kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, MCF7
Cellular locationCytoplasm, Membrane, Nucleus
Customer validation

(H460,A549, Human lung cancer, Non-small-cell carcinoma)

WB(Homo sapiens, Sus scrofa, Mus musculus, Capra hircus)

IHC(Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - CDK4 Rabbit pAb (A0366) }

Western blot - CDK4 Rabbit pAb (A0366)

Western blot analysis of extracts of various cell lines, using CDK4 antibody (A0366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - CDK4 Rabbit pAb (A0366) }

Western blot - CDK4 Rabbit pAb (A0366)

Western blot analysis of extracts of various cell lines, using CDK4 antibody (A0366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - CDK4 Rabbit pAb (A0366) }

Immunohistochemistry - CDK4 Rabbit pAb (A0366)

Immunohistochemistry of paraffin-embedded human colon using CDK4 antibody (A0366) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CDK4 Rabbit pAb (A0366) }

Immunofluorescence - CDK4 Rabbit pAb (A0366)

Immunofluorescence analysis of A-549 cells using CDK4 antibody (A0366). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CDK4 Rabbit pAb (A0366) }

Immunoprecipitation - CDK4 Rabbit pAb (A0366)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody (A0366). Western blot was performed from the immunoprecipitate using CDK4 antibody (A0366) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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