Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDK4 Mouse mAb (A0016)

Publications (2) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

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Overview

Product name CDK4 Mouse mAb
Catalog No. A0016
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Immunogen information

Immunogen Recombinant protein of human CDK4
Sequence Email for sequence
Gene ID 1019
Swiss prot P11802
Synonyms CMM3; PSK-J3; CDK4
Calculated MW 34kDa
Observed MW 34kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples A-431, HeLa, NIH/3T3, Mouse thymus
Cellular location Cytoplasm, Membrane, Nucleus
Customer validation

WB (Homo sapiens)

Research Area

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK4. (Distance between topics and target gene indicate popularity.) CDK4

* Data provided by citexs.com, for reference only.

Publishing research using A0016? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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