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CDK1 Rabbit pAb (A0220)

Reviews (4)Publications (6) Datasheet

ABclonal:Western blot - CDK1 Rabbit pAb (A0220)
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220)
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220)
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220)
ABclonal:Immunofluorescence - CDK1 Rabbit pAb (A0220)
Review
ABclonal: review for CDK1 Rabbit pAb(A0220)
Review
ABclonal: review for CDK1 Rabbit pAb(A0220)
Review
ABclonal: review for CDK1 Rabbit pAb(A0220)
Review
ABclonal: review for CDK1 Rabbit pAb(A0220)

Overview

Product nameCDK1 Rabbit pAb
Catalog No.A0220
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).
SequenceSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDE
Gene ID983
Swiss protP06493
SynonymsCDK1; CDC2; CDC28A; P34CDC2
Calculated MW27kDa/34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60, HeLa, 293T, THP-1, BT-474, Mouse thymus, Mouse spleen, Rat testis
Cellular locationCytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

(Human gastric cancer cells, Human tumor cell, HeLa, HCT116)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IP(Rattus norvegicus)

ABclonal:Western blot - CDK1 Rabbit pAb (A0220) }

Western blot - CDK1 Rabbit pAb (A0220)

Western blot analysis of extracts of various cell lines, using CDK1 antibody (A0220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220) }

Immunohistochemistry - CDK1 Rabbit pAb (A0220)

Immunohistochemistry of paraffin-embedded rat brain using CDK1 antibody (A0220) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220) }

Immunohistochemistry - CDK1 Rabbit pAb (A0220)

Immunohistochemistry of paraffin-embedded mouse spinal cord using CDK1 antibody (A0220) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220) }

Immunohistochemistry - CDK1 Rabbit pAb (A0220)

Immunohistochemistry of paraffin-embedded mouse brain using CDK1 antibody (A0220) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CDK1 Rabbit pAb (A0220) }

Immunofluorescence - CDK1 Rabbit pAb (A0220)

Immunofluorescence analysis of A549 cells using CDK1 antibody (A0220). Blue: DAPI for nuclear staining.

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