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CD86 Rabbit pAb (A1199)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - CD86 Rabbit pAb (A1199)

Western blot analysis of extracts of various cell lines, using CD86 antibody (A1199) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD86 Rabbit pAb (A1199)

Immunohistochemistry of paraffin-embedded mouse spleen using CD86 Rabbit pAb (A1199) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CD86 Rabbit pAb (A1199)

Immunofluorescence analysis of Raw264.7 cells using CD86 antibody (A1199) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for CD86 Rabbit pAb(A1199)

Overview

Product nameCD86 Rabbit pAb
Catalog No.A1199
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).
SequenceAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP
Gene ID942
Swiss protP42081
SynonymsB7-2; B7.2; B70; CD28LG2; LAB72; CD86
Calculated MW12kDa/24kDa/28kDa/31kDa/37kDa
Observed MW75KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Mouse spleen, Mouse thymus, Rat lung, Rat thymus
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

(Human cells)

WB(Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus)

FC(Mus musculus)

ICC(Homo sapiens)

ABclonal:Western blot - CD86 Rabbit pAb (A1199) }

Western blot - CD86 Rabbit pAb (A1199)

Western blot analysis of extracts of various cell lines, using CD86 antibody (A1199) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD86 Rabbit pAb (A1199) }

Immunohistochemistry - CD86 Rabbit pAb (A1199)

Immunohistochemistry of paraffin-embedded mouse spleen using CD86 Rabbit pAb (A1199) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD86 Rabbit pAb (A1199) }

Immunofluorescence - CD86 Rabbit pAb (A1199)

Immunofluorescence analysis of Raw264.7 cells using CD86 antibody (A1199) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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